一、个人简介
王淑军，女，博士，二级教授，博士生导师，江苏省海洋生物资源与环境重点实验室主任，食品科学与工程学院经理，全国“五一劳动奖章”获得者， “生物工程”江苏省“十四五”重点学科带头人，江苏省海洋科学优势学科“海洋生物资源开发利用”方向带头人。“江苏省333高层次人才培养工程”中青年科学技术带头人、江苏省“六大人才高峰”培养对象、连云港市“521工程”第一层次培养对象。加拿大滑铁卢大学访问学者，主要开展海洋生物资源开发利用、酶与酶工程以及食品加工与安全方面的研究，目前主持国家自然科学基金面上项目1项，子课题主持国家重点研发计划项目1项。主持完成国家自然科学基金项目2项、国家863子课题3项、国家科技支撑项目子课題1项，国家海洋公益项目子课题1项，省科技厅自然基金、农业支撑和社会发展项目各1项；以第一或通信作者发表SCI收录论文60多篇，出版教材1部；获得中国发明专利40件。以第一完成人获得江苏省科学技术奖三等奖1项、中国轻工业联合会科学技术发明奖三等奖1项和江苏省轻工业科学技术奖励技术发明奖一等奖1项；参与完成国家海洋科技创新奖二等奖1项。兼任中国生物化学与分子生物学学会海洋分会理事，中国微生物学会微生物教学工作委员会委员，中国生物化学与分子生物学会生物化学与分子生物技术专业委员会委员，中国湖沼学会海洋生物技术分会理事，江苏省微生物学会副秘书长和常务理事，江苏省药学会海洋药物专业委员会副主任委员、江苏省食品科学与技术学会常务理事和水产食品专业委员会负责人，江苏省轻工协会理事。
二、教学情况
承担课程：食品微生物学（本科）、高级微生物学（研究生），食品微生物学进展（研究生） ，指导的多名研究生考取厦门大学等高校的博士研究生。
三、主要研究方向
海洋微生物及其活性物质、海洋生物资源开发利用、食品生物技术
四、承担的科研项目
1. 国家自然科学基金面上项目：GH49家族右旋糖酐酶水解产物中异麦芽寡糖聚合度差异的分子机制研究（32172154，2022-2025，主持）
2. 国家重点研发计划项目：深海新型工业用酶的性能优筛和改造（2022YFC2805101，2022 -2026，子课题主持）
3. 国家自然科学基金面上项目：基于晶体结构的超嗜热古菌高温α-淀粉酶热稳定性不依赖钙离子的分子机制研究（31271929，2013 -2016，主持）
4. 国家“863计划”项目子课题：海洋右旋糖苷酶的中试及前期应用研究（2011AA09070302，2011 -2014，主持）
5. 国家自然科学基金项目：连云港近岸海域沉积物中微生物群落结构与功能的研究（40746030，2008.1-2008.12，主持）
6. 国家科技支撑计划项目子课题：长江口附近海岸带生态修复及生物资源利用技术及示范（2012BAC07B03，2012 -2015，主持）
7. 国家海洋公益性行业科研专项经费项目子课题：新型海洋低温淀粉酶规模化开发和工业应用关键技术研究（201205020-8，2012 -2015，主持）
8. 江苏省科技厅社会发展项目：海洋右旋糖酐酶产业化工程技术及酶制剂产品开发应用研究（BE2016702，2016-2019，主持）
9. 江苏省科技厅农业支撑计划项目：淀粉深加工的高温耐热酸性α-淀粉酶的开发和应用研究（BE200830094，2008 -2010，主持）
10. 国家重点研发计划深海关键技术与装备重点专项：海洋来源高附加值产业化酶的挖掘和新产品开发（2018YFC0311106，2018-2021，参加）
11.国家自然科学基金面上项目：海洋节杆菌右旋糖酐酶的结构功能解析及分子改造研究（31471719，2015 -2018，第二）
12. 江苏省产学研合作项目：核酸适配体快速检测水产病原细菌方法研究（BY2020216, 2020至-2021，主持）
13.连云港市“521工程”资助项目：耐盐海洋水产品加工专用蛋白酶制剂及活性肽制备关键技术研究（LYG06521202107，2021-2023，主持）
14. 连云港高新区重点研发计划科技项目：基于功能化核酸的三种重要水产病害弧菌快速检测关键技术研究及产品开发（ZD201918，2019-2021，主持）
五、发表主要论文
1．Jingfei Hu, Xiaohua Lai, Xudong Wu, Huanyu Wang, Nanhai Weng, Jing Lu, Mingsheng Lyu, Shujun Wang*, Isolation of a Novel Anti-Diabetic α-Glucosidase Oligo-Peptide Inhibitor from Fermented Rice Bran, Food, 2023, 12, 183.
2．Huanyu Wang, Qianru Lin, Mingwang Liu, Wen Ding, Nanhai Weng, Hao Ni, Jing Lu*, Mingsheng Lyu，Shujun Wang*，Molecular Docking and Site-Directed Mutagenesis of GH49 Family Dextranase for the Preparation of High-Degree Polymerization Isomaltooligosaccharide，Biomolecules, 2023, 13.
3．Xudong Wu, Baojie Dou, Boyan Wang, Mingwang Liu, Ruxue Shao, Jing Lu*, Mingsheng Lyu, Shujun Wang*. Improved Stability and Hydrolysates of Hyperthermophilic GH57 Type II Pullulanase from the Deep-Sea Archaeon Thermococcus siculi HJ21 by Truncation, Catalysts, 2023, 13, 453.
4．Jie Hu, Cang Wang, Hangtian Zu, Zaixi Fang, Haolong Zhou, Junlan Zhou, ShujunWang*, Mingsheng Lyu*, Nano-SiO2 inhibits the marine aquatic pathogen Vibrio parahaemolyticus, Aquaculture Reports, 2022,23,101015.
5．Xueqing Tian, Jinfei Hu, Tong Wei, Wen Ding, Qingzhen Miao, Zhe Ning, Shihui Fan, Hangjie Wu, Jing Lu, Mingsheng Lyu*, Shujun Wang*, Fast and sensitive graphene oxide-DNAzyme-based biosensor for Vibrio alginolyticus detection, Journal of Fish Diseases, 2022.
6．Xiaoyi Ma, Wen Ding, Cang Wang, Hangjie Wu, Xiaopeng Tian, Mingsheng Lyu*, Shujun Wang*, DNAzyme biosensors for the detection of pathogenic bacteria, Sensors and Actuators: B. Chemical, 2021, 331:129422.
7．Xiaoyi Ma, Cang wang, Mingcan Qin, Xiaopeng Tian, Shihui Fan, Hangtian Zu, Mingsheng Lyu*, Shujun Wang*, Rapid detection of Aeromonas hydrophila with a DNAzyme-based sensor, Food Control, 2021,123:107829.
8．Jingfei Hu, Xueqing Tian, Tong Wei, Hangjie Wu, Jing Lu, Mingsheng Lyu, Shujun Wang*, Anti-Helicobacter pylori Activity of a Lactobacillus sp. PW-7 Exopolysaccharide, Foods，2021, 10. 2453.
9．Shihui Fan, Chao Ma, Xiaopeng Tian, Xiaoyi Ma, Mingcan Qin, Hangjie Wu, Xueqing Tian, Jing Lu, Mingsheng Lyu*, Shujun Wang*, Detection of Vibrio vulnificus in Seafood With a DNAzyme-Based Biosensor, Front. Microbiol. 2021,
10．Mingcan Qin，Xiaoyi Ma，Shihui Fan，Hangjie Wu，Wanli Yan，Xiaopeng Tian，Jing Lu，Mingsheng Lyu*，Shujun Wang*, Rapid detection of Pseudomonas aeruginosa using a DNAzyme-based sensor, Food Science & Nutrition, 2021,
11．Hangjie Wu, Lide Gu, Xiaoyi Ma, Xueqing Tian, Shihui Fan, Mingcan Qin, Jing Lu, Mingsheng Lyu*, Shujun Wang*, Rapid Detection of Helicobacter pylori by the Naked Eye Using DNA Aptamers, ACS Omega, 2021, 6, 5, 3771-3779.
12．Cang Wang, Xiaoyi Ma, Jie Hu, Xiaopeng Tian, Hao Zhang, Dongxue Dong, Zaixi Fang, Mingsheng Lyu, Shujun Wang*, Jing Lu*. Nanolayered Double Hydroxide Inhibits the Pathogenicity of Vibrio parahaemolyticus, Journal of Nanomaterials, 2021.
13．Yanshuai Ding, Hao Zhang, Xuelian Wang, Hangtian Zu, Cang Wang, Dongxue Dong, Mingsheng Lyu, Shujun Wang*, Immobilization of Dextranase on Nano-Hydroxyapatite as a Recyclable Catalyst, Materials, 2021,14,130
14．Dongxue Dong, Xuelian Wang, Tian Deng, Zhe Ning, Xiaopeng Tian,Hangtian Zu, Yanshuai Ding, Cang Wang, Shujun Wang, Mingsheng Lyu*, A novel dextranase gene from the marine bacterium Bacillus aquimaris S5 and its expression and characteristics, FEMS Microbiology Letters, 2021, 368(3)
15．Zhe Ning, Dongxue Dong, Xiaopeng Tian, Hangtian Zu, Xueqing Tian, Mingsheng Lyu, Shujun Wang*, Alkalic dextranase produced by marine bacterium Cellulosimicrobium sp. PX02 and its application，Journal of Basic Microbiology,2021，
16．Tian Deng, Yanli Feng, Linxiang Xu, Xiaopeng Tiana, Xiaohua Lai, Mingsheng Lyu*, Shujun Wang*, Expression, purification and characterization of a cold-adapted dextranase from marine bacteria and its ability to remove dental plaque, Protein Expression and Purification, 2020, 174:105678.
17．Xueqin Liu, Yanli Feng, Xiaohua Lai, Tian Deng, Xin Liu, Mingsheng Lyu*, Shujun Wang*, Virgibacillus halodenitrificans ST-1 for Fermentation of Shrimp Paste and Hydrolysates of its Protease，Food Science & Nutrition，2020, 1777
18．Xin Liu, Tian Deng, Xueqin Liu, Xiaohua Lai, Yanli Feng, Mingsheng Lyu*，Shujun Wang*，Isomalto-Oligosaccharides Produced by Endodextranase Shewanella sp. GZ-7 From Sugarcane Plants, Natural Product Communications, 2020, 15(9):1-10
19．Yanli Feng, Tian Deng, Xiaohua Lai, Zhihua Feng, Mingsheng Lyu*, Shujun Wang*, Effect of Properties of Silver Nanoparticles Coated by Polar Material and the Antibacterial Activity for Marine Pathogenic Bacteria, Journal of Nanomaterials, 2020,11
20．Wei Ren, Po-Jung Jimmy Huang, Meilin He, Mingsheng Lu, Changhai Wang*, Shujun Wang*, Juewen Liu*. Sensitivity of a classic DNAzyme for Pb2+ modulated by cations, anions and buffers, Analyst, 2020, 145(4): 1384-1388.
21．Wei Ren, Yanshuai Ding, Lide Gu, Wanli Yan, Cang Wang, Mingsheng Lyu, Changhai Wang*, Shujun Wang*, Characterization and mechanism of the effects of Mg–Fe layered double hydroxide nanoparticles on a marine bacterium: new insights from genomic and transcriptional analyses, Biotechnology for Biofuels, 2019,12:196
22．Wei Ren, Le Liu, Lide Gu, Wanli Yan, Yan li Feng, Dongxue Dong, Shujun Wang*, Mingsheng Lyu, Changhai Wang*, Crystal Structure of GH49 Dextranase from Arthrobacter oxidans KQ11: Identification of Catalytic Base and Improvement of Thermostability Using Semirational Design Based on B Factors, Journal of Agricultural and Food Chemistry, 2019, 67, 4355−4366.
23．Wei Ren, Po-Jung Jimmy Huang, Meilin He, Mingsheng Lu, Shujun Wang*, Changhai Wang*, Juewen Liu*. The Two Classic Pb2+‐selective DNAzymes Are Related: Rational Evolution for Understanding Metal Selectivity, ChemBioChem, 2019, 20:1-6.
24．Lide Gu, Wanli Yan, Hangjie Wu, Shihui Fan, Wei Ren, Shujun Wang*, Mingsheng Lyu*, Juewen Liu*, Selection of DNAzymes for Sensing Aquatic Bacteria: Vibrio Anguillarum, Analytical Chemistry, 2019, 91(12):7887−7893.
25．Wanli Yan, Lide Gu, Wei Ren, Xiaoyi Ma, Mingcan Qin, Mingsheng Lyu*, Shujun Wang* Recognition of Helicobacter pylori by protein-targeting aptamers, 2019, Helicobacter，2019, 24(3):1-10.
26．Hongfei Liu, Wei Ren, Mingsheng Ly, Haifeng Li*, Shujun Wang*, Characterization of an Alkaline GH49 Dextranase from Marine Bacterium Arthrobacter oxydans KQ11 and Its Application in the Preparation of Isomalto-Oligosaccharide, Marine Drugs, 2019,17:479.
27．Xiaohua Lai, Xin Liu, Xueqin Liu, Tian Deng, Yanli Feng, Xiaopeng Tian, Mingsheng Lyu*, Shujun Wang*, The Marine Catenovulum agarivorans MNH15 and Dextranase: Removing Dental Plaque, Marine Drugs, 2019,17:592.
28．Le Liu, Yi Ding, Shu Liu, Shujun Wang, Yaowei Fang, Mingsheng Lyu*, Dextrans removal from sugarcane juice using dextranase from marine bacterium Arthrobacter oxydans KQ11, Quality Assurance and Safety of Crops & Foods, 2019, 11(1):53-59.
29．Wei Ren, Ruanhong Cai, Wanli Yan, Mingsheng Lyu, Yaowei Fang, Shujun Wang*, Purification and characterization of a biofilm-degradable dextranase from a marine bacterium, Marine Drugs, 2018, 16(2), 51-67.
30．Yi Ding, Le Liu, Yaowei Fang, Xu Zhang, Mingsheng Lyu*, Shujun Wang*, The Adsorption of Dextranase onto Mg/Fe-Layered Double Hydroxide: Insight into the Immobilization, Nanomaterials, 2018, 8(3): 173-186.
31．Wanli Yan, Lide Gu, Shu Liu, Wei Ren, Mingsheng Lyu*, Shujun Wang*, Identification of a highly specific DNA aptamer for Vibrio vulnificus using systematic evolution of ligands by exponential enrichment coupled with asymmetric PCR, Journal of Fish Diseases, 2018:41(12):1821-1829.
32．Lide Gu, Wanli Yan, Shu Liu, Wei Ren, Mingsheng Lyu*, Shujun Wang*, Trypsin enhances aptamer screening: A novel method for targeting proteins, Analytical Biochemistry, 2018,561:89-95.
33．Lide Gu, Runjhun Saran, Wanli Yan, Po-Jung Jimmy Huang, Shujun Wang*, Mingsheng Lyu*, Juewen Liu*, Reselection Yielding a Smaller and More Active Silver-Specific DNAzyme, ACS Omega, 2018, 3, 15174−15181.
34．Huaixu Cheng, Zhidan Luo, Mingsheng Lyu, Song Gao*, Shujun Wang*, The hyperthermophilic alpha-amylase from Thermococcus sp. HJ21 does not require exogenous calcium for thermostability because of high-binding affinity to calcium, Journal of Microbiology, 2017, 55(5):379–387.
35．Wei Ren, Shujun Wang*, Mingsheng Lyu, Xiaobei Wang, Yaowei Fang, Yuliang Jiao, Jianen Hu*, Optimization of four types of antimicrobial agents to increase the inhibitory ability of marine Arthrobacter oxydans KQ11 dextranase mouthwash, Chinese Journal of Oceanology and Limnology, 2016, 34(2):354-366.
36．Xiaobei Wang, Huaixu Cheng, Mingsheng Lyu, Yaowei Fang, Yuliang Jiao, Weijuan Li, Gengmao Zhao, Shujun Wang*, Dextranase from Arthrobacter oxydans KQ11-1 inhibits biofilm formation by polysaccharide hydrolysis, Biofouling, 2016, 32(10): 1223–1233.
37．Delong Wang, Mingsheng Lu, Shujun Wang*, Yuliang Jiao, Weijuan Li, Qiang Zhu, Zhaopu Liu*, Purification and characterization of a novel marine Arthrobacter Oxydans KQ11 dextranase, Carbohydrate Polymers, 2014, 106:71-76.
38．Delong Wang, Mingsheng Lu, Xiaobei Wang, Yuliang Jiao, Yaowei Fang, Zhaopu Liu*, Shujun Wang*, Improving stability of a novel dextran-degrading enzyme from marine Arthrobacter oxydans KQ11, Carbohydrate Polymers, 2014,103, 294-299.
39．Yuliang Jiao, Shujun Wang*, Mingsheng Lu, Binghua Jiao, Weijuan Li, Yaowei Fang, Shuliu，Characterization of a marine-derived dextranase and its application to the prevention of dental caries. Journal of Industrial Microbiology Biotechnology, 2014, 41(1) :17-26.
40．Ruanhong Cai, Mingsheng Lu, Yaowei Fang, Yuliang Jiao, Qiang Zhu, Zhaopu Liu*, Shujun Wang*, Screening, production, and characterization of dextranase from Catenovulum sp. Annals of Microbiology, 2014, 64:147-155.
41．Xiaobei Wang, Mingsheng Lu, Shujun Wang*, Yaowei Fang, Delong Wang, Wei Ren, Gengmao Zhao*, The atmospheric and room temperature plasma method (ARTP) on the dextranase activity and structure, International Journal of Biological Macromolecules, 2014,70:284-291.
六、获得发明专利（第一发明人）
1. 用于创伤弧菌识别的核酸适配体及筛选方法与应用，ZL201810950055.7，2022
2. 纤维化纤维微细菌PX02及其产右旋糖酐酶的方法和应用， ZL 202011533460.2，2022
3. 假节杆菌NT14及其产右旋糖酐酶的方法，ZL 202011090882.7，2022
4. 芽孢杆菌GN02及其产右旋糖酐酶的方法与用途，ZL 202011555608.2，2022
5. 来自海洋的假交替单胞菌HL9及其产磷脂酶B的方法，ZL202011323280.1，2021
6. 海洋芽抱杆菌SP-5及其右旋糖酐酶，ZL 201910739361.0，2021
7. 来自海洋的卵链菌MNH15及产酶方法与产品及应用，ZL 201910736450.X，2021
8. 单链DNA核酸适配体及其筛选方法与用途，ZL201910108955.1，2021
9. 来自海洋的芽孢菌株ZE01及其产低温淀粉酶的方法，ZL201510436666.6，2017
10. 液体海洋右旋糖苷酶复合稳定剂及其应用，ZL 2013104944239，2015
11. 海洋低温右旋糖苷酶与产酶方法及其产生菌S6-2，ZL 201010534675,6，2012
12. 一种热球菌产高温α-葡萄糖苷酶及其产酶方法，ZL 200710191828.X，2010
13. 一种嗜热球菌产高温普鲁兰酶的方法及产品，ZL 200710191827.5，2010
14. 一种热球菌属嗜热古菌产耐高温α-淀粉酶的方法及产品，ZL 200710020115.7，2009
15. 一种交替假单胞菌产低温碱性蛋白酶的方法及产品，ZL 200710020114.2，2009
七、获奖成果
1. 江苏省科学技术奖三等奖：海洋微生物新型工业酶产业化应用关键技术（2019，1/7）
2. 国家海洋科学技术奖二等奖：医学组织工程材料海洋生物胶原蛋白评价技术（2017，4/10）
3. 中国轻工业联合会科学技术发明奖三等奖：海洋微生物新型工业酶产业化应用关键技术（2019，1/6）
4. 江苏省轻工业科学技术奖励技术发明奖一等奖：新型海洋酶制剂开发关键技术与应用（2019，1/5）
5. 江苏省轻工业科学技术奖励三等奖：新型海洋热稳定不依赖钙离子的超高温耐酸淀粉酶研究（2015，1/5）
八、联系方式
邮箱: sjwang@ jou.edu.cn；电话：13961372673
